动物双歧杆菌亚种。乳酸菌XLTG11通过减轻炎症，增强肠道屏障功能，调节肠道菌群，改善抗生素相关性腹泻
动物双歧杆菌亚种。乳酸菌XLTG11通过减轻炎症，增强肠道屏障功能，调节肠道菌群，改善抗生素相关性腹泻。
Bifidobacterium animalis subsp. lactis XLTG11 improves antibiotic-related diarrhea by alleviating inflammation, enhancing intestinal barrier function and regulating intestinal flora.
发布日期 : 2022-05-26
文献类型 :论著
PMID :35616024
DOI :10.1039/d1fo04305f
作者 :Xu Baofeng , Liang Shengnan , Zhao Jiayi , Li Xuetong , Guo Jiayao , Xin Bowen , Li Bailiang , Huo Guicheng , Ma Weiwei
期刊 :FOOD & FUNCTION IF: 5.396中科院 1 区
中文摘要
抗生素相关性腹泻(AAD)是抗生素治疗中常见的副作用。在这项研究中，我们评估了动物双歧杆菌亚种的调节作用。乳酸菌XLTG11对抗生素所致小鼠肠道菌群失调所致腹泻的治疗作用。然后，两株动物双歧杆菌亚种。乳酸菌XLTG11和动物双歧杆菌亚种。给AAD小鼠灌胃乳酸BB-12。结果表明，乳酸杆菌XLTG11的恢复效果优于乳酸杆菌BB-12。乳酸杆菌XLTG11可明显降低肠道病理改变，降低肠道通透性，降低内毒素(LPS)、D-乳酸(D-LA)和二胺氧化酶(DAO)水平。此外，这两株病毒还可显著增加水通道蛋白和紧密连接蛋白的表达，抑制Toll样受体4(Toll-like Receptor 4，TLR4)/核因子-核转录因子B(NFFB-κB，NF-κB)信号通路的激活，显著提高抗炎细胞因子水平，降低促炎细胞因子水平。此外，乳酸杆菌XLTG11处理后，醋酸、丙酸、丁酸和总短链脂肪酸的含量显著增加。与MC组相比，乳杆菌XLTG11增加了肠道菌群的丰富度和多样性，改变了肠道菌群组成。我们发现乳酸杆菌XLTG11可以促进肠道菌群和粘膜屏障功能的恢复，从而有效地改善AAD相关症状，为今后的临床应用提供科学依据。
英文摘要
Antibiotic-associated diarrhea (AAD) is a common side effect during antibiotic treatment. In this study, we evaluated the regulatory effect of Bifidobacterium animalis subsp. lactis XLTG11 on mouse diarrhea caused by antibiotic-induced intestinal flora disturbance. Then, two strains of Bifidobacterium animalis subsp. lactis XLTG11 and Bifidobacterium animalis subsp. lactis BB-12 were administered to AAD mice. We found that the recovery effect of using B. lactis XLTG11 was better than that of B. lactis BB-12. B. lactis XLTG11 reduced the pathological characteristics of the intestinal tract, and significantly reduced the levels of lipopolysaccharide (LPS), D-lactic acid (D-LA) and diamine oxidase (DAO) to decrease intestinal permeability. In addition, these two strains significantly increased the expression of aquaporin and tight junction proteins, and inhibited toll-like receptor 4 (TLR4)/activation of the nuclear factor-κB (NF-κB) signaling pathway, significantly increased the levels of anti-inflammatory cytokines and decreased levels of pro-inflammatory cytokines. Moreover, after treatment with B. lactis XLTG11, the contents of acetic acid, propionic acid, butyric acid and total short-chain fatty acids were significantly increased. Compared with the MC group, B. lactis XLTG11 increased the abundance and diversity of the intestinal flora and changed the composition of the intestinal flora. We found that B. lactis XLTG11 can promote the recovery of intestinal flora and mucosal barrier function, thereby effectively improving AAD-related symptoms, providing a scientific basis for future clinical applications.
